29F-28 |
Synergistic inhibition effect of Korean propolis and Cordyceps militaris on food spoilage microorganisms |
Y. R. SON, J. Y. Han, D. R. Yoo, and S. B. Nam. Research & Development Center, Haitai Confectionery & Food Co., Ltd., 255-9, Huam-Dong, Yongsan-Ku, Seoul, 140-190, South Korea Approved chemical preservatives have been widely used to preserve foods and increase their shelf life. There are increasing demends of the partial or complete removal of chemical preservatives from foods because of adverse health effect of chemicals. In this study, the possibility of natural antimicrobial compounds, Korean propolis and extracts of Cordyceps militaris as food preservatives are investigated. Propolis sample was collected in Kang-Won province, South Korea and extracted with 70% ethanol. Liquid culture of Cordyceps militaris was extracted by 80% ethanol. The antimicrobial activities of propolis extracts and extracts of Cordyceps militaris against several food spoilage microorganisms were examined. Escherichia coli, Bacillus cereus, Bacillus subtilis, Salmonella typhimurium, Staphylococcus aureus, Fusarium oxysporum and Fusarium sporotrichioides as food poisoning microorganisms were chosen. When the extracts was added to food product, antimicrobial effect was tested during storage at 4£§C. Propolis extracts and extracts of Cordyceps militaris had antimicrobial activity against food spoilage microorganisms. When the microorganisms were treated with propolis extracts, the population of food spolige microorganisms decresed by 2.6-3.7 log cycles. And the polulations of microorganisms treated with extracts of Cordyceps militaris reduced by 2.1-3.2 log cycles. The combination use of propolis extracts and extracts of Cordyceps militaris (1:1) showed a synergistic effect on the inhibition of microorganisms. The inhibitory effect of combination use of extracts increased in more acidic condition. The combination of use of extracts in food products, Korean Mandu (Korean traditional food) was effective at preventing microbial growth and extending shelf-life. The combination use of propolis extracts and extracts of Cordyceps militaris were more effective than single use. Therefore, it can be possible to apply combination use of propolis extracts and extracts of Cordyceps militaris in several food products as a natural food preservative.
Session 29F, Food Microbiology: Control of foodborne microorganisms by antimicrobials
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