14E-34 |
Safety evaluation and antioxidative effect of Cynanchum wilfordii Hemsley extracts in rats |
M. K. Sung, Food and Nutrition, Sookmyung Women`s University, 53-12, Chungpa-Dong 2-ga, Yongsan-gu, Seoul, 140-742, South Korea, H. S. KWON, Agr/Product Activation Office, National Agricultural Cooperative Federation, 75 Chungjungro 1-ga, Junggu, Seoul, 100-707, South Korea, and E. J. Lee, Food and Nutrition, Sookmyung Women's University, 53-12 Chungpa-dong 2-ka, Yongsan-ku, Seoul, 140-742, South Korea. Cynachum wilfordii Hemsley has been used for centuries as a tonic neuraceutical in China and Korea. This plant has been known to possess an advantage of anti-aging effect, which possibly is related to its antioxidative capacity. Oxidative modification of LDL has been recognized as playing an important role in the initiation and progression of atherosclerosis by forming fatty streak and foam cells. Therefore, antioxidants from natural substances may decrease LDL susceptibility to oxidation and thereby preventing cardiovascular disease. However, the increased use of natural products created an important issues, safety. The purpose of this study was to evaluate 1)the subchronic toxicities of Cynanchum wilfordii Hemsley in rats, to determine the likely toxicity for humans, and 2)effect of extracts on LDL oxidation induced by Cu2+ and lipid peroxide formation in rats. Cynanchum wilfordii Hemsley was powered, and extracted with ethylacetate or water. Rats were gavaged with either 5mg/100g bw/day(ethylacetate) or 7.5mg/100g bw/ day(water) for 13 wks, to test subchronic toxicity. To evaluate the efficacy against plasma LDL-oxidation and tissue lipid peroxide formation, rats were fed a high fat diet with or without extract administration. Results suggest that no observable toxic effect was found based on hematology and blood chemistry. LDL oxidation and tissue lipid peroxide were increased in groups fed the high fat diet. But there was no significant difference in LDL oxidation between control and extract-fed groups. However, rats fed the HF+extract showed a significantly lower levels of tissue lipid peroxide compared to those in the control rats. These results suggest that Cynanchum wilfordii Hemsley may be a safe material to reduce in vivo oxidative stress, although it did not significantly inhibit LDL-oxidation. This research was supported by Ministry of Agriculture and Forestry, Korea
Session 14E, Nutraceuticals & Functional Foods: General I
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