14E-22 |
Free radical scavenging activity of Dulse, Palmaria palmata extract |
Y. V. YUAN1, D. Bone, M. Carrington, and K. Harrison. (1) School of Nutrition, Ryerson University, 350 Victoria St., Toronto, ON M5B 2K3, Canada Edible seaweeds such as Laminaria and Porphyra sp. have been demonstrated to have potential beneficial health effects such as reducing the risk of chronic diseases such as breast or colon cancer in animal studies. One mechanism in support of this effect is thought to be the antioxidant activity of various seaweeds observed in vitro. The objective of the present study was to characterize the antioxidant activity of Dulse, Palmaria palmata using a variety of in vitro methods. Methanol extracts of freeze-dried Dulse were evaluated for the ability to inhibit lipid oxidation and scavenge the free radicals 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) and 2,2'-azo-bis(2-amidinopropane) dihydrochloride (AAPH). As well, the Dulse extracts were evaluated for reducing activity and the ability to chelate metal ions. The Dulse extract inhibited production of conjugated dienes (233 nm) and thiobarbituric acid reactive substances (TBARS) as measures of lipid peroxidation in a linoleic acid emulsion incubated at 38°C. Similarly, the Dulse extract exhibited free radical scavenging activity against the radical cation ABTS and inhibited lipid peroxidation initiated by AAPH in vitro. The Dulse extract exhibited reducing activity, but did not possess copper chelation activity in vitro. In the present study, the methanol extract of Dulse was able to inhibit the oxidation of long-chain polyunsaturated fatty acids in an emulsion as well as exhibit free radical scavenging activity against AAPH and ABTS systems in vitro. The mechanisms of antioxidant activity of the methanol extract of Dulse, Palmaria palmata, likely involve reducing activity, but not transition metal ion chelation.
Session 14E, Nutraceuticals & Functional Foods: General I
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