14E-29 |
Phenolic antioxidants from mulberry (Morus Albba L.) fruits with inhibitory activity against angiotensin-converting enzyme and xanthine oxidase |
Y. J. CHO1, S. S. Chun2, and W. S. Cha1. (1) Dept. of Food Engineering, Sangju National Univ., 386 Gajangdong, Sangju, Gyeongbuk, 742-711, South Korea, (2) Dept. of Food Science, Univ. of Massachusetts, Amherst, Chenoweth Lab., Box 31410, Amherst, MA 01003-1410 Natural antioxidants are widely used because they are considered safer and have been linked with fewer adverse reactions. Many phenolic compounds from plant are good sources of natural antioxidants. Some phenolics are also responsible for biological activity such as anti-hypertension and anti-cancer etc. The objective of this research was to determine the phenolic profiles related antioxidant activity of mulberry (Morus alba L.) fruits extracts, and evaluate the inhibitory activity against angiotensin-converting enzyme (ACE) and xanthine oxidase (XOase) lead to hypertension and gout. Water and ethanol extracts from mulberry fruits were prepared. Total phenolics were assayed using the Folin-ciocalteu method. Phenolic profiles in mulberry fruits extracts were analyzed using HPLC. Antioxidant activities were measured by 1,1-diphenyl-2-picrylhydrazyl-2-radical (DPPH) method, antioxidant protection factor (PF) method using b-carotene, 3-ethyl benzthiazoline-6-sulfonic acid (ABTS+) method and tiobarbituric acid reaction substance (TBARS). Inhibitory activity against ACE and XOase were determined spectrophotomertrically. Total phenolics were the highest in 60% ethanol extracts of mulberry fruits. DPPH radical scavenging activity depended on the kind of phenolic compound and percentage inhibition activity (ABTS+) and hydroxyl radical scavenging activity depended on amount of total phenolics. TBARS and PF were related to lipophilic compounds in mulberry fruits. Ethanol extracts from mulberry fruits showed inhibitory activity against ACE and XOase. It was the highest in 60% ethanol extracts. The results indicate that phenolics from mulberry fruits could use as natural antioxidant and the source of anti-hypertension and anti-gout.
Session 14E, Nutraceuticals & Functional Foods: General I
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