14E-32 |
Protection of butanol fraction of methanol extract from Aster scaber against in the oxidative damage in the brain of mice administered with kainic acid |
M. R. KIM1, D. E. SOK2, and S. Oh1. (1) Department of Food & Nutrition, Chungnam National University, 220 Gung-Dong, Yuseong-Ku,Taejon 305-764, KOREA, Taejon, 305-764, South Korea, (2) College of Pharmacy, Chungnam National University, 220 Gung-Dong, Yuseong-Ku, Taejon, 305-764, South Korea Oxidative stress is increased during aging and in neurodegenerative disorders. Oxidant radicals are implicated as a causal factor in the pathogenesis of neurobiological disorders and neurodegenerative diseases. Concerned with this, there have been reports concerning the prevention by antioxidants against the oxidative damage in brains subjected to inducers of oxidative stress such as kainic acid. Meanwhile, there are few studies on the protective action of extracts of Aster scaber against oxidative damage in brain tissue. The objective of this study is to investigate the protective effect of butanol fraction from methanol extract from Aster scaber on the oxidative damage in the brain of mice administered with kainic acid. After butanol fraction (0.4 or 1.0 g/kg) of each Aster scaber was administered to ICR male mice, 6 to 8 weeks, through a gavage for 4 days consecutively, and on the 3 rd day, kainic acid (50 mg/kg) was i.p. administered, behavioral sign and biochemical parameters of oxidative stress such as lipid peroxidation(TBARS), total glutathione amount, glutathione peroxidase activity and glutathione reductase activity was examined. When compared to the vehicle-treated control, no significant changes in body and brain weight were observed in mice administered with butanol fraction of Aster scaber extract. When butanol fraction of each Aster scaber extract was examined for the neuroprotective action, the butanol fraction of Aster scaber, (0.4 g/kg) alleviated the lethality (25 %) of kainic acid, and the behavioral sign of its neurotoxicity. Moreover, the administration of the butanol fraction of Aster scaber extract at doses of 0.4 g/kg and 1 g/kg restored the glutathione level in the cytosolic portion of brain homogenate to approximately 80 and 110 % (p<0.05), respectively. Also, the butanol fraction of Aster scaber extract (0.4 g/kg) led to a significant reduction of kainic acid-induced increase of TBARS value. These results suggest that the butanol fraction of Aster scaber extract is a functional agent to prevent against neurotoxicity.
Session 14E, Nutraceuticals & Functional Foods: General I
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