14E-12 |
Cytoprotective effect of polysaccharide isolated from mushrooms against 7-ketocholesterol induced damages in mouse liver cell line (BNL CL.2) |
J. S. KIM1, K. Na2, K. N. Kim1, and H. Y. Chung3. (1) Pulmuone R&D Center, Pulmuone Co., Ltd., Seodaemun PO Box 146, Seodeamun-Ku, Seoul, 120-600, South Korea, (2) Pulmuone R&D Center, Pumuone Co., Ltd., Seodaemun PO Box 146, Seodeamun-Ku, Seoul, 120-600, South Korea, (3) Biology Dept., Food and Nutritional Sciences Programme, and Food Sci. Lab., The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, Hong Kong (1) Justification 7-Ketocholesterol oxidized at C-7 position has been identified as a main component of oxidized low-density lipoprotein (oxLDL) and is strongly cytotoxic to endothelial cells resulting in cell death due to apoptosis. Polysaccharides of natural products have been reported to prevent oxidative damage in cell lines. Several essential biological activities and anti-complementary properties of polysaccharides are well known including antitumor, immunological effect, anti-inflammatory, and hypoglycemic defect. However, there are no studies in the hepatoprotective activity of mushroom polysacchride isolates on oxysterol-induced liver cell damage. We hypothesize that polysaccharides isolated from mushrooms may inhibit oxLDL-induced cytotoxicity in a normal liver cell line. (2) Objectives The purpose of this research was to evaluate the cytoprotective activity of polysaccharides isolated from six different edible mushrooms on 7-ketocholesterol-induced damaged cell line. (3) Methods Polysaccharides from six different edible mushrooms-Flammulina velutipes, Peurotus ostreatus, Lentinus edodes, Agrocybe aegerita, Agaricus blazei, and Cordyceps militaris-were isolated by hot water extraction and alcohol precipitation. Cytoprotective acitivity was evaluated by measuring the viable cells of a normal embryonic liver cell line (BNL CL. 2) in the presence of 7-ketocholesterol at different concentrations (0, 20, 40, and 80 mg/mL) during a 20-h incubation period. Viable cells in the cell line were determined by the mitochondrial dehydrogenase activity using MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide) and by trypan blue exclusion assay. (4) Results At 80 mg/mL of 7-ketocholesterol, cytotoxicity was very high with a loss of 98% viable cell after 20-h incubation. With the addition of 200 mg/mL of each polysaccharide isolate to 80 mg/mL 7-ketocholesterol, polysaccharide isolates from both Flammulina velutipes and Peurotus ostreatus significantly inhibited 7-ketocholesterol-induced cytotoxicity after 20-h incubation whereas other polysaccharide isolates were not significantly different when compared to a control (p<0.05). (5) Significance This study showed the effectiveness of mushroom polysaccharide isolates in inhibiting cell damage caused by oxLDL-induced cytotoxicity.
Session 14E, Nutraceuticals & Functional Foods: General I
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