14E-8 |
Antioxidant and antimutagenic properties of Phaseolus vulgaris and Phaseolus coccineous black seeded bean varieties |
F. G. LOARCA-PIÑA1, H. Guzmán-Maldonado2, J. Acosta-Gallegos2, and S. García-Delgado3. (1) Programa de Posgrado en Alimentos del Centro de la República, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas S/N, 76010, Querétaro, 76010, Mexico, (2) Biotechnology and Legume Laboratories, Experimental Station El Bajío, National Research Institute for Forestry, Agriculture and Livestock (INIFAP), Km 6 Carretera Celaya-San Miguel de Allende, Celaya, Celaya, Guanajuato, 38000, Mexico, (3) 1Programa de Posgrado en Alimentos del Centro de la República (PROPAC), Faculta de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas S/N, Queretaro, Qro, 76010, Mexico Dry beans are an integral part of diets in Latin America and Africa, but the potential benefits of consuming beans from a nutraceutical standpoint have largely been overlooked. Seed coat of dry beans is rich in phenolic compounds. Phenolic compounds have shown to be effective antioxidants, antimutagens and anticarcinogens and have shown anti-atherosclerotic properties as well. To evaluate the antioxidant and antimutagenic properties of polyphenols from seed coat of Phaseolus vulgaris and Phaseolus coccineous black seeded varieties. Total phenolic compounds were quantified in seed coats of P. vulgaris and P. coccineous according to Deshpande and Cheryan (1985, 1987). The antioxidant potential was evaluated using beta-carotene-linoleate and 1, 1-dhiphenyl-2-picrylhydrazyl (DPPH) in vitro model system. To examine the effect of phenolic compounds against aflatoxin B1 (AFB1) mutagenicity, the microsuspension assay with tester strains TA100 and TA98, was used. Beans were pearled and methanolic extracts of pearled beans sample exhibited antioxidant activity by beta-carotene-linoleate that correlated with phenolic content and inhibited DPPH significantly in a dose-dependent manner. The antimutagenic effect of bean phenols against AFB (1) correlated with phenolic content and was also dose-dependent. The results suggested that bean phenols can use as antioxidant and antimutagenic agents. Common bean could be considered as a nutraceutical and functional food.
Session 14E, Nutraceuticals & Functional Foods: General I
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