18-5 |
Validation of an efficient method for the determination of catechins in green tea leaves, extracts, and fortified products using high-performance liquid chromatography (HPLC) |
Z. LILLA1, S. Habeck, and D. Levin. (1) Covance Laboratories, 3301 Kinsman Blvd., Madison, WI 53527 Catechins are a family of polyphenol compounds that are found predominantly in green tea. These phytochemical compounds have been shown to have antioxidant properties and studies have indicated a link to a wide range of health benefits including a reduced risk of cancer, and heart disease as well as antimicrobial properties. Interest in the functional properties of these compounds has resulted in the analysis and development of catechin containing products. To support product development and clinical studies, an efficient, cost-effective method for quantitating the content of these compounds in teas, tea extracts, and fortified products was needed. Six compounds: catechin, epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), epigallocatechin gallate (EGCG), and gallocatechin gallate (GCG) were isolated utilizing reversed-phase high-performance liquid chromatography (HPLC) and quantified with ultraviolet (UV) detection. By modifying and optimizing chromatographic parameters, a gradient method was established that would enable simultaneous quantitation of all six compounds within a single aliquot analyzed by HPLC thereby enabling the determination of catechin for multiple analyses over a short period of time. Typical relative standard deviations on various matrices investigated are less than 2%. The increasing interest in the beneficial properties of phytochemicals is playing a significant role to the food and dietary supplement industries. The identification of catechins as a component of consumer and scientific interest necessitates the rapid evaluation and quantitation of these compounds in food and dietary products. Validation of this HPLC method is a key factor in the providing dependable data to support scientific and regulatory decisions.
Session 18, Chemistry and analysis of phytochemicals or plant bioactive materials
|