14E-3 |
A rapid electroanalytical tool for identification of nutraceutical lignans (Sesamin, Sesamolin, Sesamol, Sesaminol, Sesamolinol) in sesame (Sesamicum Indicum L.) and tahina |
O. TOKUSOGLU, S. Aycan, and S. Kocak. Dept. of Chemistry, Celal Bayar Univ., Akhisar M.Y.O., Manisa, 45200, Turkey Recently, there has been increasing interest to nutraceuticals in dietary foods due to their protective biochemical functions and antioxidant effects. The need for regular monitoring of nutraceuticals in functional foods and food products has led to an increasing demand for adequately sensitive and selective analytical techniques with simultaneous determination capabilities. Differential Pulse Polarography (DPP) has become accepted as one the most powerful electroanalytical tool for minor components in foods and biological matrices due to its extreme sensitivity and selectivity. Sesame (Sesamum Indicum L.) sample (Muganlï) grown during the summer period of 2002 was obtained from Ege Agricultural Research Institute, Menemen and dry-milled into flours prior electroanalytical analysis. Tahina samples were collected from Altan Manisalï A.S., Koska A.S., Ömer Bese A.S. manufacturer. A polarographic analyzer (METROHM 746 VA Trace Analyzer) together with a capillary hanging mercury drop electrode (HMDE) was used for all electroanalytical measurements. A platinum wire was used as the counter electrode and Ag/AgCl was the reference electrode. HP 5400/5973 model gas chromatograph using a 30 m, 0.25 mm, 0.25 µm capillary column HP-5-MS (J & W Scientific, Folsom, CA) was used for Gas-Chromatography Mass Spectrometry (GC-MS). Antioxidant lignans (Sesamin, Sesamolin, Sesamol, Sesaminol, Sesamolinol) in sesame, marketed formulations of tahina and halva which is processed tahina food were determined by Differensial Pulse Polarography (DPP). Samples have been analysed by standard addition procedures and found to be quantitative (p<0.01). Data was confirmed by GC-MS analysis. Major potent phenolic lignan was sesamin and the linear domain range was 728.8-729.3 mg/100g oil for sesamin (R2=0.9999) (p<0.01) in unroasted sesame sample. The proposed Differensial Pulse Polarographic (DPP) method is a rapid, reproducible for simultaneous determination of phenolic lignans in sesame and food products with sesame. It provides an adequate, sensitive quantitative dedection of these nutraceuticals in commercial food industry.
Session 14E, Nutraceuticals & Functional Foods: General I
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