61A-50 |
Detection of irradiated carbohydrates and honey using FT-Raman and FTIR spectroscopy |
R. KIZIL and J. M. K. Irudayaraj. Dept. of Agricultural & Biological Engineering, Pennsylvania State Univ., 108 Agricultural Engineering Bldg., University Park, PA 16801 Irradiation as a food preservation method and detection of irradiated food have increasing attraction. The objective of the study was to investigate the use of vibrational spectroscopic technique for rapid identification of irradiated carbohydrates such as fructose, beet invert, cane invert and corn syrup as well as honey. Samples were irradiated at doses of 1, 5, 10, and 17 kGy using cobalt source gamma rays. The Fourier-Transform infrared and Raman spectra of the samples were obtained using the Nicolet 870 FT-Raman/FTIR dual bench with an InGAs and a DTGS detectors. The carbohydrate samples were characterized based on their vibrational band characteristics and biochemical changes due to irradiation was identified. A multivariate statistical method, combination of partial least square with canonical variate analysis (CVA), was developed based on the variations in spectra of irradiated and normal carbohydrates. Separation of irradiated carbohydrates from the native (non-irradiated) carbohydrates was obtained in discriminant space using spectral information obtained from the skeletal vibration mode of pyranose ring, O-H and C-H stretch region. The findings suggest the potential of vibrational spectroscopy as a tool to detect the qualitative changes in irradiated carbohydrate systems
Session 61A, Carbohydrate
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