15C-20 |
Isolation and characterization antibacterial compound from Kaempferia pandurata against oral pathogens |
J. H. Park1, K. M. Park, J. Y. Jung, N. I. Baek4, J. E. Kim, and J. K. HWANG6. (1) Department of biomaterials science and engineering, Yonsei University, Yonsei engineering research complex B118CA, Seoul, 120-749, South Korea, (2) Department of life science, Kyunghee University, Yongin, Seoul, South Korea, (3) Bioproducts Research Center, Yonsei University, B138A Yonsei Engineering Complex, 134 Shinchon-dong Sudaemun-gu, Seoul, 120-749, South Korea
The occurrence of dental caries was mainly associated to oral pathogens, especially Streptococcus mutans. Although various antiseptics and antibiotics are used as treatment for dental caries, recent attempt has been conducted to search for natural products due to their side effects. Our preliminary antibacterial screening for medicinal plants showed that the extract Kaempferia pandurata, widely cultivated in Thailand and Indonesia for the spicy flavour, possessed strong inhibitory activity against Streptococcus mutans. The purpose of this study was to identify and characterize the anticariogenic compound isolated from Kaempferia pandurata. The methanol extract of Kaempferia pandurata was tested for in vitro antibacterial activity against Streptococcus mutans. According to the bactericidal test, 0.005% of the methanol extract mostly inactivated S. mutans in 1 min, and the activity was much higher than that of green tea extract. The methanol extract was then fractionated with ethyl acetate, butanol and water, and the ethyl acetate fraction exclusively exhibited antibacterial activity. An active antibacterial compound from the ethyl acetate fraction of Kaempferia pandurata was purified to a single compound using TLC analysis and silica gel column chromatography. The active antibacterial compound of Kaempferia pandurata against S. mutans was identified as isopanduratin A (C26H30O4) by 13C-NMR, 1H-NMR and EI-MS. Minimum inhibitory concentration (MIC) value was determined as 4 งถ/งข, which was much lower than those of other natural anticariogenic agents such as tea polyphenol, carvacrol, thymol and sanguinarine. Isopanduratin A also exhibited antibacterial potential against Porphyromonas gingivals, S. sobrinus, S. aureus and S. sangius. The results strongly suggested that isopanduratin A could be employed as a potential anticariogenic agent in functional foods and oral care products.
Session 15C, Food Chemistry: Food composition and analysis
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