46E-23 |
Effect of different molecular size of porphyran on the growth of murine macrophage |
H. S. SHIM1, S. K. Seul2, H. J. Seo1, D. S. Byun1, T. S. Shin3, H. R. Kim1, and S. B. Kim1. (1) Faculty of Food Science & Biotechnology, Pukyong National Univ., College of Fisheries Science, Busan, 608-737, South Korea, (2) Dept. of Biology, Pusan National Univ., College of Natural Science, Busan, 609-735, South Korea, (3) Dept. of Food Science & Nutrition, Yosu National Univ., Yosusi, chunnam, Yosu, 550-749, South Korea Porphyra, a red algae, is plentifully cultivated in Japan, China, and Korea. Dried laver is made from Porphyra yezoensis. Porphyran is distributed in the intercellutar matrix and cell wall of red algae and its basic structure is closely related to agarose. The primary structure of porphyran shows alternating 1,4-linked 3,6-anhydro-L-galactose units and 1,3-linked b-D-galactose units, which sometimes replace to L-galactose-6-sulfate and 6-O-methyl derivatives, respectively. Porphyran has been showing physiological functionality such as improvement of microflora in the colon, antitumor activity, antihypertensive effect, and macrophage stimulation activity. The objective was to establish the extraction condition of porphyran from laver and to investigate which molecular size of porphyran has more effect on the activation of immune cells. Porphyran was extracted from Porphyra yezoensis with different pH, temperature, and time. The extracted porphyran was fractionated with hollow fiber type ultramembrane filter (MWCO; 10, 30, 50 and 100 kDa). Murine macrophage was incubated for 5 days with different molecular size of porphyran with different concentration (0.1-1000 µg/mL). Cell proliferation was assayed by Trypan blue staining and cytoxicity was measured by MTT assay. Cytokines were assayed by ELISA and Western blotting. Optimum extraction conditions for the extraction of porphyran from laver were estimated to be at pH 1.0 and temperature 60oC for 3 hr agitation in 40 volumes of solvent to dry weight. Cell proliferation was significantly increased in the addition of 10 §¶/§¢ of porphyran lower than 10 kDa and higher than 100 kDa of molecular mass (p<0.05). Cell proliferation was also increased by the addition of 1 §¶/§¢ of porphyran higher than 100 kDa of molecular mass (p<0.05). These results suggest that extraction conditions for porphyran from laver will be applicable for the production of porphyran from the red algae. Macrophage proliferation will contribute on the commercialization of laver products.
Session 46E, International
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