46F-2 |
Cirsium Maackii Maxim extract reduces liver damages and lipids in rats induced by CCl4 and high fat |
Y. B. OH1, M. E. Jung1, H. S. Ryu2, and C. K. Chung1. (1) Dept of Food and Nutrition, Hallym Univ., Okchon-dong, Chunchon, 200-702, South Korea, (2) Faculty of Food Sci. and Biotechnology, Bukyong Nat'l Univ., Daeyeon-dong, Busan, 608-737, South Korea Cirsium Maackii Maxim(CMM) leaf is an edible mountain vegetable in Korea. It is known to have an effect on fever, jaundice, hypertension and enteritis. The objective of this study was to examine the effect of CMM on the liver damage and lipid metabolism in rats given CCl4 and high fat. Male Sprague Dawley(SD) rats were divided into five groups consisting the control(C), high fat fed group(HF), high fat and CMM fed group(HFCM), CCl4 treated group(CC), CCl4 and CMM fed group(CCCM). To induce liver damage, the mixture(1ml/Kg body weight) of CCl4 and olive oil (1:1) was i.p. injected. The mixture was given twice a week for 3 weeks at 8th day of the experiment. High fat diet containing 10% lard and 0.1% sodium cholate was given with experimental diet. Fresh high pressure extracted CCM was given daily with drinking water. Weight of the liver, kidney, spleen was generally decreased by CMM. Serum total cholesterol was decreased by 42.3% in HFCM group compared to HF group. Feeding of the extract resulted in the decrease of serum triglyceride by 35.3% in high fat group. On the other hand, HDL was increased by 22.8%. Total liver lipid was significantly decreased by CMM. Total liver cholesterol also was decreased by 18.2%. In CCCM group, cholesterol was decreased by 48.9% and triglyceride was decreased by 47.2% compared to CC group. Representative liver enzymes, alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were decreased by the extract. In CCl4 groups, ALT was decreased by 23.94% with the extract feeding. Ultrastructural examination of hepatocytes of the CCl4 administered rats showed increased lipid droplets and damaged lesions. However, hepatocytes of those fed in combination with the extracts were shown to be reduced in damages of the liver along with reduced droplets in size. On the basis of the above results, Cirsium Maackii Maxim is believed to have a possible protective effect for the high fat-induced and carbon tetrachloride-induced hepatotoxicity in rats.
Session 46F, Nutrition
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