61A-25 |
The effect of irradiation on chitosan and alginate |
W. S. CHOI and H. J. Park. Graduate School of Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-ku, Seoul, 136-701, South Korea Chitosan and alginate monomer and oligomer derived from radiation-induced degradation of chitosan and alginate were determined by gel permeation chromatography (GPC) and high performance liquid chromatography (HPLC). They have been widely used in paper, medicine, food and cosmetic field. The objectives of this research were 1) to investigate the effect of irradiation on chitosan and alginate in various dose ranges and 2) to determine chitosan and alginate monomer and oligomer degraded by irradiation The chitosan and alginate were irradiated in aqueous solution with Co-60 gamma rays in the dose ranges of 10 -200 KGy to investigate the degradation effect of radiation on chitosan and alginate polymer. The chitosan and alginate monomer and oligomer were determined by GPC and HPLC After irradiation, the degradations of alginate and chitosan in water and 2% acetic acid solution each were determined. In case of chitosan, molecular weights were considerably decreased with radiation dose increased from 0 KGy to 100 KGy, but there was a little decrease in molecular weight with radiation dose increased from 100 KGy to 200 KGy. The D-glucosamine hydrochloride, dimer, trimer, tetramer, pentamer, hexamer of chtosan were determined by HPLC and the total amount of chtosan oligomer (1 - 6) was ca. 15% for 100 KGy irradiation. It is certified that radiation can provide a useful tool for degradations of chitosan or alginate and lower the cost of chitosan or alginate monomer and oligomer production.
Session 61A, Carbohydrate
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