30G-5 |
Determination of triglyceride composition of vegetable oils using HPLC and evaporative light scattering detection |
J. W. WALSH, Product Development, Alltech Associates, Inc., 2051 Waukegan Road, Deerfield, IL 60015 and R. Gaita, LC/IC Instrument Support, Alltech Associates, Inc, 2051 Waukegan Rd., Deerfield, IL 60015. Edible fats and oils are primarily composed of triglycerides. Vegetable oils possess a unique pattern of triglycerides that can be used to determine origin and detect adulteration. In food products that contain significant levels of oil, the behavior of the product and its quality can depend on the triglyceride composition of the oil. For these reasons, it is clearly important to have a good method of analysis for triglycerides. Our objective was to develop a single, rapid reversed-phase HPLC method using Evaporative Light Scattering Detection (ELSD). A gradient, reversed-phase HPLC method was used for the separation of triglycerides in the vegetable oils. For detection of the triglycerides we used the ELSD. The ELSD is able to detect any sample that is less volatile than the mobile phase and is gradient compatible. Our results showed that a single, fast (less than 15 minutes) reversed-phase HPLC method can be used for the analysis of all the various vegetable oils. This method can be used to efficiently determine the origin and detect adulteration of vegetable oils.
Session 30G, Fruit & Vegetable Product: Processed Fruits and Vegetables
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