46E-7 |
Study of the growth of Pleurotus ostreatus on supplemented substrates |
H. HERNÁNDEZ-SÁNCHEZ, Depto. Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, Col. P.E. Calles, México, CP. 11340, Mexico and M. M. Flores-Sánchez. Supplemented substrates used at the fructification level, are known to increase the yield and biological efficiencies in Pleurotus ostreatus. Therefore, the use of supplemented media to produce spawns of this mushroom could increase the degradation effect on the substrate and also the yield, productivity, and biological efficiency at different production stages of this mushroom. The purpose of this research was then to study the growth of P. ostreatus in solid culture media and grain spawn supplemented with: amaranth flour (AF), deffated soy flour (DSF) and soy husks (SH) at five main levels: 1, 3, 5, 10 and 15%. Fructification in unsupplemented straw with the obtained spawns was also studied The growth was evaluated by measuring the diameter of the colony in solid culture media and by determining the chitin content in the case of the spawns. In the fructifications, the weight of the whole mushroom was recorded. Yield and productivity were also calculated. The highest apparent productivity (9.7 mm/day) was obtained in the case of 15% AF supplemented yeast and malt extract agar. In the case of wheat spawns, the highest yield (362.6 mg of chitin/g of grain after 17 days) and productivity (21.3 mg/g/day) was obtained with the 1% AF supplemented grains. The fructification experiments indicated that the highest biological efficiency (65%), yield (19.5 g of mushroom / 100 g of straw) and productivity (0.368 g of mushroom/ 100 g of straw/ day) were obtained when the 1% SH supplemented spawns were used to inoculate the barley straw. These results suggest that the use of AF could be recommended to obtain a good growth on solid media and spawn. However, soy husks due to its very low cost are strongly recommended for supplementing spawn to obtain better results in the fructification stage.
Session 46E, International
|