15E-1 |
Anti-photoaging effect of dietary soy isoflavones in a hairless mouse model |
S. Y. KIM, N. Y. Kim, W. G. Kim, H. W. Park, Y. C. Sim, and S. J. Lee. Pharmaceutical & Health Research Institute, Amore Pacific Corporation R&D Center, Kiheung-eup, Yongin-si, Kyounggi-do, South Korea In recent years, isoflavones have obtained increased attention because of their health-related beneficial aspects. Isoflavones are estrogen-like compounds from dietary source that are associated with a broad range of biological activities due to their hormonal and antioxidant action. Despite lots of reports linking the beneficial properties of isoflavones to degenerative disease, no studies have been conducted investigating the effect of isoflavones on skin and skin fibroblast cells. Our objective was to investigate protective effects of dietary isoflavones against UV irradiation in a hairless mouse model. Hairless mice were orally administered with soy extract(500mg/Kg body Wt/d) containing isoflavones(30%) and were exposed to UV-irradiation three times a week over one month to afford a total dose of 600mJ/cm2. At the end of the study, it was measured the effects of isoflavones on skin appearance, collagen deposition and epidermal thickness in the UV-damaged mouse skin. In skin fibroblast culture, the effects of isoflavones on synthesis/degradation of collagen and metalloproteinases(MMPs) were determined. Our results showed better skin appearance and less wrinkles in isoflavone treated group compared to UV-damaged, untreated group. Also, skin roughness parameter values(Ra, Rm, Rt) measured by skin visiometer were significantly decreased in isoflavone group. In addition, histological analysis of type 1 pN collagen showed that isoflavone treatment resulted in increased collagen deposition in UV-damaged mouse skin dermis. In fibroblast culture, significantly decreased MMP1 secretion(46.2%) was observed in isoflavone group compared to UVB-irradiated, untreated group. Taken together the results, it seems that increased collagen fibers in isoflavone administered hairless mouse skin is due to the inhibitory effect of isoflavones on the collagen breakdown in UV-irradiated skin. Our study suggests that dietary isoflavones may have photoprotective effect on UV-damaged skin.
Session 15E, Nutraceuticals & Functional Foods I
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