61A-3 |
Hydrolysis of sugar cane bagasse with hydrochloric acid at atmospheric pressure to obtain xylose |
G. Bustos1, J. A. Ramírez2, and M. VÁZQUEZ2. (1) Dept. of Food Science, Univ. Autonoma de Tamaulipas, UAM Mante, Ciudad Mante, Tamaulipas, 89840, Mexico, (2) Dept. of Food Science & Technology, Univ. Autonoma de Tamaulipas, UAM Reynosa-Aztlán, Reynosa, Tamaulipas, 88700, Mexico Sugar cane bagasse is an important agricultural waste in Mexico. The hydrolysis of sugar cane bagasse eliminates a waste and generates a value-added product, xylose. The xylose obtained from the hemicellulosic hydrolysis of sugar cane bagasse can be used for its conversion through chemical of biotechnological processes to xylitol. The hydrolysates contain mainly sugars such as xylose and glucose but also furfural and acetic acid which can be growth inhibitors. The objective of this work deals with the hydrolysis of sugar cane bagasse using hydrochloric acid for obtaining fermentable xylose solutions at atmospheric pressure (100 ºC). Kinetic models were developed to explain the time course of xylose, glucose, acetic acid and furfural generated. The raw material used in experiments was sugar cane bagasse collected in a local industry. Sugar cane bagasse was air dried, milled, screened to select the fraction of particles with a size lower than 0.5 mm, homogenized in a single lot and stored until needed. Moisture was determined gravimetrically, xylose, glucose and acetic acid were determined by HPLC with RI detector, and furfural was determined by UV-Vis spectrometry. Analyses of the main fractions (cellulose, hemicelluloses and lignin) were carried out using a quantitative acid hydrolysis under standard conditions. Treatments were performed at 100ºC in media containing 2, 4 or 6 % HCl. Samples were collected at several reaction times in the range 0-300 min. The potential concentration of xylose (corresponding with the quantitative conversion of xylan to xylose without degradation reactions) was 23.4 g/L. The maximum xylose concentration was obtained using 6% HCl and 300 min, which corresponding with 22.3 g xylose/L. In these conditions, it was also obtained 4.13 g glucose/L, 4.14 g acetic acid/L and 1.09 g furfural/L. The results obtained showed that HCl can be used in the hydrolysis of sugar cane bagasse.
Session 61A, Carbohydrate
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