44F-30 |
Tocopherols extraction from soybean oil deodorizer distillate by saponification: the effect of acetone/water solution composition |
M. E. M. ALMEIDA1, O. Rusig2, and E. C. Guzman2. (1) Centro de Tecnologia de Hortifrutícolas, –ITAL-Instituto de Tecnologia de Alimentos, CP 139, Campinas , SP, 13073-001, Brazil, (2) Departamento de Tecnologia de Alimentos, Faculdade de Engenharia de Alimentos-UNICAMP, Caixa Postal 6121, Campinas - SP, 13083-970, Brazil The distillate of soybean oil deodorization (DDSO) is used as a raw material to obtain tocopherol concentrates or vitamin E. The tocopherol extraction obtained from that very complex mixture of components, needs a combination of various techniques such as physical and chemical treatments. The main objective of this research was to study the influence of acetone/water composition in the tocopherols extraction using a saponification method. Solutions of DDSO in concentrations of 5, 10, 15, 20 and 25% (w/w) have been applied in acetone/water mixture with acetone proportions of 100, 98, 95, 92,5, 85 and 80% (v/v). After the saponification process using Ca(OH)2 (15g/L) the DDSO solutions were cooled down to 00C, centrifuged and the rich in tocopherols supernatant phase, collected for further determinations. Tocopherols were analyzed by HPLC according to AOCS(1993). The DDSO used has shown the following composition (g/100g): total tocopherols (9.50), a-tocopherol (1.3), b+g-tocopherol (5.9) and d-tocopherol (2.3). This investigation showed that for all acetone concentrations used, those assays resulting in concentrates with higher tocopherol content showed a lower tocopherol recovery from DDSO. The use of solvents plus acetone in levels ranging from 90-95% and DDSO from 20-20%, resulted in tocopherol concentration twice as high as the other treatments. The best result was reached for a 92.5% of acetone solution and 25% DDSO resulting a concentrate with the following composition (g/100g): total tocopherols (26.0), a-tocopherol (3.3), b+g -tocopherol (16.0) and d-tocopherol (6.5). This method may be applied in process to pre-concentrate tocopherols for further purification treatment. The results also indicate the need of optimization study based on acetone and DDSO concentration range from 90-92.5% and 20-25% respectively.
Session 44F, Product Development
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