30A-24 |
Lipase-catalyzed production of structured lipids from borage oil and eicosapentaenoic acid (EPA) |
F. SHAHIDI and S. P. J. N. Senanayake. Department of Biochemistry, Memorial University of Newfoundland, St. John's, NF A1B 3X9, Canada Borage oil is an excellent source of g-linolenic acid (GLA; 18:3n6). GLA has been used in the treatment of certain skin-related disorders as well as a variety of other diseases. On the other hand, eicosapentaenoic acid (EPA; 20:5n3) has potential for prevention of cardiovascular disease. Therefore, a structured lipid containing both GLA and EPA may be of interest because of its desired health benefits. The objective of this study was to optimize the reaction conditions for incorporation of EPA into borage oil by lipase from Pseudomonas sp. using response surface methodology (RSM). The reaction variables examined were the amount of enzyme (150-350 U), incubation temperature (20-60oC) and incubation time (6-30 h). Up to 35.4% EPA was incorporated into borage oil under optimum reaction conditions over a 27 h period. Meanwhile, the GLA content in the oil decreased from 23.5 to 11.0%. Analysis of variance (ANOVA) showed that 99% (R2=0.99) of the observed variation was explained by the polynomial model. The reproducibility of the model was satisfactory as coefficient of variation was only 2.9. The final oil had a ratio of n-6 to n-3 of 1:1 which is nutritionally more favorable than the original unmodified oil. The structured lipid containing GLA and EPA, produced successfully in this study, may be used in certain nutritional and therapeutic applications.
Session 30A, Food Chemistry: Lipids
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