44E-14 |
Lupinus campestris seeds as a source of nutraceutical foods |
G. DÁVILA ORTÍZ1, C. Jiménez Martínez1, G. F. Loarca-Piña2, N. Robledo Quintos3, J. Martínez Herrera3, and M. E. Jaramillo-Flores. (1) Dept. de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas - IPN, Prolongación de carpio y Plan de Ayala, Col. Casco de santo Tomas, México, DF, 11340, Mexico, (2) Dept. de Investigación y Posgrado, Univ. Autónoma de Queretaro, Facultad de Química, Queretaro, QRO, Mexico, (3) Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Yautepec Morelos, Mexico Lupin seeds represent a potentially important source of protein for animal and human consumption. The plant shows advantages such tolerance of poor soil quality, adaptability to temperature and climate. High protein content (300-450 g/kg-1) and high oil content (10-20 g/kg-1)in the seed of some species. However, the utilization of this crop has been limited by the presence of toxic alkaloids (AQ) and other antinutritional factors such as a-galactosides (Gal) and phenolic compounds (PC). Other authors suggest this compounds present antimutagenic and anticarcinogenic activity. On other hand, nitroarenes are present in diesel and gasoline emissions, ash particles, cigarrette smoke condensates, home heater emissions and the urban atmospheres. Nitroarenes such as 2-nitrofluorene (2-NF) and 1-Nitropyrene (1-NP) are potent mutagens towards Salmonella typhimurium strains. The objetive this study was to quantify and examine the effect of quinolizidinic alkaloids, a-galactosides and phenolic compounds from Lupinus campestris seeds on 1-NP mutagenicity, using the microsuspension assay test. Isolation (Wink, 1994) and quantification of QA by GC/SM, Gal for HPLC (Macrae and Zand Moghadam, 1987) and PC by colorimetric method (Singleton, 1968). The mutagenic activity of 1-NP and antimutagenic activity were determined using the microsuspension assay test on tester strain YG1024 . The obtained results showed that L.campestris seeds have 28.8g/kg-1 of QA, 97.5 g/kg-1 of G and 24.39 g/kg-1 of EF. The inhibition percentage of 1-NP mutagenicity were 76% (13.6 mg/plate), 76.31% (512 mg/plate) and 87% (200 mg/plate) respectively. These results suggest that L. campestris seeds do not only represent a protein source, beside have substances with pharmacological properties and it can be used in the elaboration of nutraceutical foods.
Session 44E, Nutraceuticals & Functional Foods
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