44E-13 |
Inhibition of high fat-induced oxidative stresses in rats by Cynanchum wilfordii hemesley |
H. S. KWON1, Y. I. Na2, and M. K. Sung2. (1) Institute for Agricultural Food Technology, National Agricultural Cooperative Federation, 38-27, Wondang-Dong, Goyang-Si, Kyongki-Do, 412-707, South Korea, (2) Food and Nutrition, Sookmyung Women`s University, 53-12, Chungpa-Dong 2-ka, Yongsan-ku, Seoul, 140-742, South Korea
The occurrence of degenerative chronic diseases such as cancer and cardiovascular heart disease is closely related to oxidative stresses. Many plant antioxidants have been proposed to suppress oxidative stresses in biological systems. The objective of this study was to evaluate antioxidative effects of Cynanchum wilfordii hemesley, which have been used as anti-aging plants for many years in China and Korea. To accomplish this objective, the freeze-dried and powdered sample of Cynanchum wilfordii hemesley was fractionated using different organic solvents. Each fraction was tested for their in vitro electron-donating ability (EDA) and inhibitory effect on malondialdehyde formation. The fraction possessing the most potent antioxidative capacity was used to examine in vivo effects. Forty male Sprague Dawley rats were divided into 4 diet groups: control, high-fat, control + extract, and high fat + extract. The Cynanchum wilfordii hemesley extract was gavaged (100mg/kg) every other day for 6 weeks. Malondialdehyde content and superoxide dismutase activity in the blood and liver of animals were measured. Results showed that ethylacetate fraction of Cynanchum wilfordii hemesley possessed highest antioxidative activity and the concentration of 1.0mg/ml showed 86% EDA and inhibited malondialdehyde formation by 55% in vitro. In vivo results exhibited that plasma and liver malondialdehyde content of animals fed high fat + extract was significantly reduced compared to those of animals fed high fat diet without the extract. Also, the activity of SOD in the plasma and liver of animals fed high fat diet + extract was significantly increased by 9% and 50%, respectively, compared to those of animals fed high fat diet without the extract. These results indicate the ethylacetate fraction of Cynanchum wilfordii hemesley reduce high fat diet induced oxidative stresses, thereby may decrease the risk of chronic disease.
Session 44E, Nutraceuticals & Functional Foods
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