88D-1 |
Reduction of biogenic amines by bioprotective cultures in raw chicken breast inoculated with Enterobacteriaceae and Pseudomonads |
B. QUINTERO-SALAZAR1, L. Pérez-Chabela2, I. Guerrero2, and E. Ponce-Alquicira2. (1) Biotecnología, Universidad Autónoma Metropolitana, Av. Purísima y Michoacan, Col. Vicentina Apartado postal 55 535, México D.F, 09340, Mexico, (2) Mexico Several microorganisms including pathogens and spoilage flora contaminate poultry flesh. Some microorganisms produce biogenic amines by decarboxylation of free amino acids. Amines are related to food poisoning and are consider as spoilage indicators. Selected lactic acid bacteria (LAB) has been suggested as bioprotective cultures for preventing the growth of spoilage flora including proteolytic and decarboxylating strains. The objective was to study the effect of selected LAB on biogenic amine production in poultry inoculated with Pseudomonads and Enterobacteriaceae. Portions of chicken breast were inoculated by immersion in a cell suspension (ODl600=1) of L. lactis ATCC 11454 or S. carnosus Chr. Hansen MC-1-02055 mixed with indicators P. fluorescens C65 or E. coli ATCC 8937, respectively. Controls were prepared as before without inoculatung LAB. Samples were vacuum packaged and stored at 10°C for eight days, and analyzed for Pseudomonads, and Enterobacteriaceae populations using GSP and RVB media, respectively. Bigenic amines were extracted with trichloroacetic acid, derivatized with benzoyl chloride and analyzed by HPLC. As expected, populations of Pseudomonads and Enterobacteriaceae were higher in control samples (p=0.005). After 8 days of storage only putrescine and cadaverine were detected in all samples; samples with no LAB had higher amine concentrations (p=0.005). Putrescine and cadaverine concentrations in control samples with E. coli were 31.2 and 58.4 mg/kg, respectively. Concentration of the same amines in samples inoculated with Pseudomonads were 34.0 and 75.14mg/kg, respectively. Levels of putrescine and cadaverine in samples with S. carnosus were lower than 13.3 and 30 mg/kg, respectively. However, in samples inoculated with L. lactis putrescine was not detected while cadaverine concentration was similar to those for samples inoculated with S. carnosus. A high correlation was observed between biogenic amines and both, E. coli and Pseudomonads, populations. LAB significantly decreased levels of cadaverine and putrescine in poultry inoculated with Enterobacteriaceae and Pseudomonads.
Session 88D, Food Microbiology: General
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